Journal: Respiratory Research
Article Title: Pharmacologically-induced mitotic synchrony in airway epithelial cells as a mechanism of action of anti-inflammatory drugs
doi: 10.1186/s12931-015-0293-4
Figure Lengend Snippet: VBP15 effectively reduces post-wounding asthmatic epithelial basolateral inflammatory secretions: Levels of cytokines measured by cytometric bead assay are shown for basolateral secretions from asthmatic and non-asthmatic human airway epithelial cells differentiated at air-liquid interface. Measurements were obtained post-injury at +24, and +48 h. Data are shown as mean ± SEM in pg/mL and significance was obtained by t -test using corresponding time points for DMSO vehicle (VEH)-exposed, DEX-exposed, and VBP15-exposed asthmatic cultures. Experiments were conducted in triplicate using N = 3 donors. (* p < 0.05 vs. vehicle, # p < 0.05 vs. DEX)
Article Snippet: The airway epithelial cells were maintained at 37 °C for 7 days and then placed at air-liquid interface with proprietary basolateral media (provided by the manufacturer; MatTek Corp.) lacking glucocorticoids and epidermal growth factor.
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